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staining with dapi  (Dojindo Labs)


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    Structured Review

    Dojindo Labs staining with dapi
    Staining With Dapi, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 484 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining+solution/Cellstain+DAPI+solution/pm42120415-827-18-21
    Average 96 stars, based on 484 article reviews
    staining with dapi - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Protective effects of coenzyme Q 10 on cell damage induced by hydrogen peroxides in cultured skin fibroblasts
    Article Snippet: .. After the fibroblasts on each well of 24-well plate were washed with PBS twice, the cells were incubated with DAPI staining solution (Dojindo Laboratories) for 30 min in CO 2 incubator, fluorescence of DAPI-DNA complex meaning cell death was observed by using fluorescence microscopy (IX-71, Olympus). ..

    Article Title: Genetic landscape and ligand-dependent activation of sonic hedgehog-Gli1 signaling in chordomas: a novel therapeutic target.
    Article Snippet: Chordoma, a rare neoplasm derived from intraosseous notochordal remnants, is unresponsive to conventional chemotherapy and radiotherapy.. Sonic Hedgehog (Shh) is a crucial fetal notochord–secreted morphogen that directs notochordal development.. The aim of this study was to determine the functional roles and therapeutic potential of Shh-Gli1 signaling in chordomas.

    Article Title: Gluconeogenesis during development of the grass puffer (Takifugu niphobles).
    Article Snippet: During the development of teleost fish, the sole nutrient source is the egg yolk.. The yolk consists mostly of proteins and lipids, with only trace amounts of carbohydrates such as glycogen and glucose.. However, past evidence in some fishes showed transient increase in glucose during development, which may have supported the development of the embryos.

    Staining:

    Article Title: Protective effects of coenzyme Q 10 on cell damage induced by hydrogen peroxides in cultured skin fibroblasts
    Article Snippet: .. After the fibroblasts on each well of 24-well plate were washed with PBS twice, the cells were incubated with DAPI staining solution (Dojindo Laboratories) for 30 min in CO 2 incubator, fluorescence of DAPI-DNA complex meaning cell death was observed by using fluorescence microscopy (IX-71, Olympus). ..

    Article Title: Transcription-coupled changes in genomic region proximities during transcriptional bursting
    Article Snippet: .. The cells were then stained for 30 s at room temperature with a DAPI staining solution (4× SSCT solution with DAPI at 1:100 dilution; Dojindo, 340-07971). .. Last, an antibleaching buffer containing 50 mM tris-HCl (pH 8.0), 300 mM NaCl, 2× SSC, 3 mM trolox (Sigma-Aldrich, 238813), 0.8% d -(+)-glucose (Nacalai Tesque, Kyoto, Japan, 16806-25), 1000-fold diluted catalase (Sigma-Aldrich, C3155), and glucose oxidase (0.5 mg/ml; Sigma-Aldrich, G2133) was added before imaging via microscopy.

    Article Title: Genetic landscape and ligand-dependent activation of sonic hedgehog-Gli1 signaling in chordomas: a novel therapeutic target.
    Article Snippet: Chordoma, a rare neoplasm derived from intraosseous notochordal remnants, is unresponsive to conventional chemotherapy and radiotherapy.. Sonic Hedgehog (Shh) is a crucial fetal notochord–secreted morphogen that directs notochordal development.. The aim of this study was to determine the functional roles and therapeutic potential of Shh-Gli1 signaling in chordomas.

    Article Title: Gluconeogenesis during development of the grass puffer (Takifugu niphobles).
    Article Snippet: During the development of teleost fish, the sole nutrient source is the egg yolk.. The yolk consists mostly of proteins and lipids, with only trace amounts of carbohydrates such as glycogen and glucose.. However, past evidence in some fishes showed transient increase in glucose during development, which may have supported the development of the embryos.

    Article Title: Transcription-coupled changes in higher-order genomic structure and transcription hub viscosity prolong enhancer-promoter connectivity
    Article Snippet: .. The cells were then stained for 30 seconds at room temperature with a DAPI staining solution (4×SSCT solution with DAPI at 1:100 dilution; Dojindo, 340-07971). .. Finally, an anti-bleaching buffer containing 50 mM Tris-HCl pH 8.0, 300 mM NaCl, 2×SSC, 3 mM trolox (Sigma-Aldrich, 238813), 0.8% D-(+)- Glucose (Nacalai Tesque, Kyoto, Japan, 16806-25), 1,000-fold diluted catalase (Sigma-Aldrich, C3155), and 0.5 mg/ml glucose oxidase (Sigma-Aldrich, G2133) was added prior to imaging via microscopy.

    Fluorescence:

    Article Title: Protective effects of coenzyme Q 10 on cell damage induced by hydrogen peroxides in cultured skin fibroblasts
    Article Snippet: .. After the fibroblasts on each well of 24-well plate were washed with PBS twice, the cells were incubated with DAPI staining solution (Dojindo Laboratories) for 30 min in CO 2 incubator, fluorescence of DAPI-DNA complex meaning cell death was observed by using fluorescence microscopy (IX-71, Olympus). ..

    Microscopy:

    Article Title: Protective effects of coenzyme Q 10 on cell damage induced by hydrogen peroxides in cultured skin fibroblasts
    Article Snippet: .. After the fibroblasts on each well of 24-well plate were washed with PBS twice, the cells were incubated with DAPI staining solution (Dojindo Laboratories) for 30 min in CO 2 incubator, fluorescence of DAPI-DNA complex meaning cell death was observed by using fluorescence microscopy (IX-71, Olympus). ..



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